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Research
1. Detection and identification of
salmonella species in minced beef and chicken meats by using
Multiplex PCR in Assiut city - Raafat
Hassanein, Sohaila Fathi Hassan Ali, Ashraf Mohamed Abd El-Malek,
Moemen, A. Mohamed, and Khalid Ibrahim Elsayh
Veterinary World, 2011, 4(1) : 5-11
doi:
10.5455/vetworld.2011.5-11
Abstract
The present study was undertaken to determine the incidence
and distribution of Salmonella species in selected meat and
chicken products purchased from retail supermarkets in Assiut,
Egypt. A total of 75 samples including 25 samples each of
minced frozen beef, frozen chicken legs and frozen chicken
fillets were collected over a 7-month period between January
and July 2009 and examined for the presence of Salmonella
species. In addition, 28 children stool cultures were
collected from hospitalized children resident in Pediatric
University Hospital with diarrhea or fever. Out of the total
75 meat samples examined, Salmonella was detected in 5 (20%)
of minced frozen beef, 9 (36%) of frozen chicken leg and 13
(52%) of frozen chicken fillet samples analyzed. Regarding the
examined 28 children stool cultures, 3 (10.71 %) were found
Salmonella positive. Of the total 30 Salmonella positive
samples from all examined samples, five selected Salmonella
isolates were further identified using multiplex PCR (m-PCR).
Two serovars were the dominant serovar identified was
Salmonella entrica subsp. entrica serovar Enteritidis (2
chicken leg isolates and 2 chicken breast fillets) followed by
Salmonella entrica subsp. entrica serovar Kentucky (one minced
beef isolate). The public health hazards of Salmonella were
discussed and the suggestive measures to protect the consumers
and improve the quality of meat and chicken products were
given.
Keywords: Salmonellosis, Minced beef, Chicken legs,
Chicken breast fillet, Incidence, m-PCR, Food borne illness,
Zoonosis.
